HPLC Column, RP C4 Butyl, Wide Pore, 10um, 4.6mm ID x 150mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C4 Butyl Wide Pore is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional butyl (C4) ligand bonding and complete double endcapping. Classified as USP L26, this stationary phase combines moderate hydrophobic retention with a 300 Å pore structure that improves access to the bonded phase for larger molecules. The C4 chemistry is particularly effective for proteins, peptides, enzymes, and other biomolecules that benefit from reduced hydrophobic interaction compared to longer-chain alkyl phases.
68004-15P-3M is a 150 mm x 4.6 mm ID analytical HPLC column packed with 10 µm RP C4 Butyl Wide Pore particles featuring a 300 Å pore size. The 150 mm column length provides increased resolving power and peak capacity compared to shorter columns, making it well suited for biomolecule characterization, impurity assessment, and method optimization. The 10 µm particle size allows operation at higher flow rates with lower backpressure, while the 4.6 mm ID format is the industry standard for conventional HPLC systems and typically operates at approximately 1.0–2.0 mL/min.
This USP L26 column is designed for the separation and characterization of proteins, peptides, enzymes, and other large hydrophobic or neutral compounds. The combination of fully endcapped C4 chemistry, high-purity silica, wide pore architecture, and longer column length provides improved resolution, excellent analyte recovery, and reliable chromatographic performance for demanding bioanalytical and biomolecule-focused applications.
Key Benefits
RP C4 Butyl Wide Pore stationary phase
USP L26 classification
Fully double endcapped chemistry
Traditional butyl (C4) bonded phase
300 Å wide pore structure optimized for large molecules
High-purity silica support
10 µm particles for lower operating backpressure
Suitable for higher flow rates
Enhanced resolution from 150 mm column length
Excellent for protein and peptide separations
Typical Applications
Reversed-phase HPLC
USP L26 methods
Protein analysis
Peptide analysis
Enzyme characterization
Bioanalytical methods
Biomolecule separations
Method development
Impurity investigations
Large molecule analysis
Specifications
Product Type: HPLC Column
Stationary Phase: RP C4 Butyl Wide Pore
USP Classification: L26
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 4.6 mm ID x 150 mm
Length: 150 mm
Recommended Flow Rate: 1.0–2.0 mL/min
Brand: Cogent
Important Notes
Designed for reversed-phase chromatography of proteins and other large molecules
Wide pore structure improves analyte accessibility and mass transfer
Provides less hydrophobic retention than comparable C8 columns
Larger particle size reduces operating backpressure
Longer column length enhances separation of closely related biomolecules
Compatible with conventional HPLC systems
Alternative for many USP L26 methods